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Validation of Transfected C2C12 Murine Myoblast Cells with Chrimson Channelrhodopsin

Biological machines enable the discovery of natural and non-natural emergent behaviors. One non-natural behavior is optogenetic function which allows for light-mediated control of skeletal muscle cells. In conjunction with 3D printing, these modified skeletal muscle cells may be used towards the development of a soft robot that actuates in the presence of light. The addition of optogenetic components such as Chrimson channelrhodopsin ion channels enable the creation of a system that can be stimulated using red light. However, the transfection must be assessed to verify the initial health and function of the cell line was unaffected when adding the optogenetic capabilities. This study focuses on evaluating the transfection efficiency of red shifted Chrimson channelrhodopsin in murine skeletal muscle cells.

Alexa Avecilla
University of Florida
Biomedical Engineering
Research Advisor: 
Dr. Rashid Bashir
Department of Research Advisor: 
Year of Publication: