Phthalates are manufactured chemicals used as plasticizers to make plastics more flexible or durable. Roughly 100% of the US population has measurable exposure amounts to phthalates. Phthalates can be found in nearly any product containing plastics, which includes personal care products, cosmetics, children's toys, and household products. The widespread use of phthalates in consumer products raises a public health concern. Phthalates can be absorbed via inhalation, absorption, ingestion, and parenteral administration. Women and children are more likely to be exposed to higher amounts of phthalates due to increased use of personal care products and phthalates commonly found in indoor dust, building materials, and flooring. This project will help address key concerns about the reproductive effects of phthalate exposure and their impact on future generations. The question being tested is does prenatal exposure to an environmentally relevant phthalate mixture causes transgenerational effects in F3 female mice? To test this question, we will focus on one major aim. 1) Determining if prenatal exposure to an environmentally relevant phthalate mixture alters DNA methylation by causing a change in the expression of enzymes responsible for methylating and demethylating DNA.